Article
Assessing the role of alkane hydroxylase genotypes in environmental samples by competitive PCR.
Journal of applied microbiology - 1 Jan 2005
Heiss-Blanquet S, Benoit Y, Maréchaux C, Monot F
Abstract excerpt
AIMS: A molecular tool for extensive detection of prokaryotic alkane hydroxylase genes (alkB) was developed. AlkB genotypes involved in the degradation of short-chain alkanes were quantified in environmental samples in order to assess their occurrence and ecological importance. METHODS AND RESULTS: Four primer pairs specific for distinct clusters of alkane hydroxylase genes were designed, allowing amplification...
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