Capping of U6 small nuclear RNA in vitro can be uncoupled from transcription.

Article

Capping of U6 small nuclear RNA in vitro can be uncoupled from transcription.

The Journal of biological chemistry - 5 Jun 1990

Gupta S, Singh R, Reddy R

Abstract excerpt

U6 small nuclear RNA (snRNA) is a required component in the splicing of eukaryotic pre-mRNAs. Mammalian U6 snRNA was synthesized in vitro by T7 RNA polymerase and purified on polyacrylamide gels. This U6 RNA, with pppG on its 5' end, was accurately capped to CH3-O-pppG, when incubated with HeLa c...

Topics

Animals
Guanine
HeLa Cells
Humans
Methylation
Mice
Mutation
Phosphates
RNA Caps
RNA, Small Nuclear
T-Phages
Transcription, Genetic

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