Article
The fission yeast Pvg1p has galactose-specific pyruvyltransferase activity.
FEBS letters - 2 Apr 2013
Yoritsune Ken-ichi, Matsuzawa Tomohiko, Ohashi Takao, Takegawa Kaoru
Abstract excerpt
N-Glycan from the fission yeast Schizosaccharomyces pombe contains outer-chain pyruvic acid 4,6-ketal-linked galactose (PvGal). Here, we characterized a putative S. pombe pyruvyltransferase, Pvg1p, reported to be essential for biosynthesis of PvGal. When p-nitrophenyl-β-Gal (pNP-β-Gal) was used as a substrate, the structure of the recombinant Pvg1p product was determined to be pNP-PvGal by one- and...
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