Article
High-resolution melting molecular signatures for rapid identification of human papillomavirus genotypes.
PloS one - 1 Jan 2012
Lee Ta-Hsien, Wu Tzong-Shoon, Tseng Ching-Ping, Qiu Jiantai Timothy
Abstract excerpt
BACKGROUND: Genotyping of human papillomarvirus (HPV) is crucial for patient management in a clinical setting. This study accesses the combined use of broad-range real-time PCR and high-resolution melting (HRM) analysis for rapid identification of HPV genotypes. METHODS: Genomic DNA sequences of 8 high-risk genotypes (HPV16/18/39/45/52/56/58/68) were subject to bioinformatic analysis to select for appropriate PCR...
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