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Article

Pulse-chase analysis for studies of MHC class II biosynthesis, maturation, and peptide loading.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

Hou Tieying, Rinderknecht Cornelia H, Hadjinicolaou Andreas V, Busch Robert, Mellins Elizabeth

Abstract excerpt

Pulse-chase analysis is a commonly used technique for studying the synthesis, processing and transport of proteins. Cultured cells expressing proteins of interest are allowed to take up radioactively labeled amino acids for a brief interval ("pulse"), during which all newly synthesized proteins incorporate the label. The cells are then returned to nonradioactive culture medium for various times ("chase"), during...

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PMID: 23329504
DOI: 10.1007/978-1-62703-218-6_31
PMCID: PMC4159250

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