Article
Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production.
Journal of bioscience and bioengineering - 1 Jun 2013
Kubo Yuji, Inaoka Takashi, Hachiya Tsuyoshi, Miyake Masayuki, Hase Sumitaka, Nakagawa Rikio, Hasegawa Hiromasa, Funane Kazumi, Sakakibara Yasubumi, Kimura Keitarou
Abstract excerpt
The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the β-subunit of the RNA polymerase.
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