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Article

A gene expression screen.

Proceedings of the National Academy of Sciences of the United States of America - 15 Dec 1991

Wang Z, Brown D D

Abstract excerpt

A gene expression screen identifies mRNAs that differ in abundance between two mRNA mixtures by a subtractive hybridization method. The two mRNA populations are converted to double-stranded cDNAs, fragmented, and ligated to linkers for polymerase chain reaction (PCR) amplification. The multiple cDNA fragments isolated from any given gene can be treated as alleles in a genetic screen. Probability analysis of the...

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Identifiers

PMID: 1722336
DOI: 10.1073/pnas.88.24.11505
PMCID: PMC53164

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