Article
Cloning of full-length cDNA sequence of a novel candidate gene related to mouse cleft palate.
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Oct 2005
Li Xin, Jin Yan, Yan Wei, Liu Yuan, Wang Xin-wen
Abstract excerpt
OBJECTIVE: Cloning and screening a novel candidate gene related to developing mouse cleft palate. METHODS: The differentially expressed genes were cloned by modified PCR-based subtractive hybridization. After identification using reverse dot blotting, positive clones were sequenced and analyzed for homology in GenBank databases. RESULTS: Four novel express sequence tags were obtained, one of which spanning 809 bp...
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