Article
Cloning of full-length cDNA sequence of a novel candidate gene related to mouse cleft palate.
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Oct 2005
Li Xin, Jin Yan, Yan Wei, Liu Yuan, Wang Xin-wen
Abstract excerpt
OBJECTIVE: Cloning and screening a novel candidate gene related to developing mouse cleft palate. METHODS: The differentially expressed genes were cloned by modified PCR-based subtractive hybridization. After identification using reverse dot blotting, positive clones were sequenced and analyzed for homology in GenBank databases. RESULTS: Four novel express sequence tags were obtained, one of which spanning 809 bp...
Topics
- Adaptor Proteins, Signal Transducing
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Cleft Palate
- Cloning, Molecular
- DNA, Complementary
- Female
- Genetic Predisposition to Disease
- Mice
- Mice, Inbred C57BL
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
- Pregnancy
- Sequence Analysis, DNA