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Article

The selective isolation of novel cDNAs encoded by the regions surrounding the human interleukin 4 and 5 genes

1 Jan 1992

Abstract excerpt

We have developed modifications to direct cDNA selection that allow the rapid and reproducible isolation of low abundance cDNAs encoded by large genomic clones. Biotinylated, cloned genomic DNAs are hybridized in solution with amplifiable cDNAs. The genomic clones and attached cDNAs are captured on streptavidin coated magnetic beads, the cDNAs are eluted and amplified. We have applied this protocol to a 425kb YAC...

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PMID: 1408833
DOI: 10.1093/nar/20.19.5173

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