Article
A sensitive real-time PCR based assay to estimate the impact of amino acid substitutions on the competitive replication fitness of human immunodeficiency virus type 1 in cell culture.
Journal of virological methods - 1 Apr 2013
Liu Yi, Holte Sarah, Rao Ushnal, McClure Jan, Konopa Philip, Swain J Victor, Lanxon-Cookson Erinn, Kim Moon, Chen Lennie, Mullins James I
Abstract excerpt
Fixation of mutations in human immunodeficiency virus type 1 (HIV-1), such as those conferring drug resistance and immune escape, can result in a change in replication fitness. To assess these changes, a real-time TaqMan PCR detection assay and statistical methods for data analysis were developed to estimate sensitively relative viral fitness in competitive viral replication experiments in cell culture. Chimeric...
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