Article
TolC and DsbA are needed for the secretion of STB, a heat-stable enterotoxin of Escherichia coli.
Molecular microbiology - 1 Oct 1995
Foreman D T, Martinez Y, Coombs G, Torres A, Kupersztoch Y M
Abstract excerpt
STB secretion-deficient mutants were isolated using the synthetic transposon Tn beta laM. Cultures were plated using a double-membrane system of cellulose acetate and nitrocellulose placed on Luria agar plates containing carbenicillin. The STB bound to the underlying nitrocellulose membrane was detected with anti-STB antibodies. The altered genes of two STB secretion-deficient mutants were identified by...
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