Article
The effect of strand bias in Illumina short-read sequencing data.
BMC genomics - 24 Nov 2012
Guo Yan, Li Jiang, Li Chung-I, Long Jirong, Samuels David C, Shyr Yu
Abstract excerpt
BACKGROUND: When using Illumina high throughput short read data, sometimes the genotype inferred from the positive strand and negative strand are significantly different, with one homozygous and the other heterozygous. This phenomenon is known as strand bias. In this study, we used Illumina short-read sequencing data to evaluate the effect of strand bias on genotyping quality, and to explore the possible causes...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.