Article
Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae.
Genome biology - 1 Jan 2005
Eide David J, Clark Suzanne, Nair T Murlidharan, Gehl Mathias, Gribskov Michael, Guerinot Mary Lou, Harper Jeffrey F
Abstract excerpt
BACKGROUND: Nutrient minerals are essential yet potentially toxic, and homeostatic mechanisms are required to regulate their intracellular levels. We describe here a genome-wide screen for genes involved in the homeostasis of minerals in Saccharomyces cerevisiae. Using inductively coupled plasma-atomic emission spectroscopy (ICP-AES), we assayed 4,385 mutant strains for the accumulation of 13 elements (calcium,...
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