Detection of mutations and DNA polymorphisms using whole genome Southern Cross hybridization.

doi:10.1093/nar/18.4.809

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Article

Detection of mutations and DNA polymorphisms using whole genome Southern Cross hybridization.

Nucleic acids research - 25 Feb 1990

Ruvkun G, Gilbert W, Horvitz H R

Abstract excerpt

We report a general method for the detection of restriction fragment length alterations associated with mutations or polymorphisms using whole genomic DNA rather than specific cloned DNA probes. We utilized a modified Southern Cross hybridization to display the hybridization pattern of all size-s...

Topics

Animals
Autoradiography
Blotting, Southern
Caenorhabditis
Chromosome Deletion
Cloning, Molecular
DNA
Genes
Mutation
Myosins
Nucleic Acid Hybridization
Phosphorus Radioisotopes
Polymorphism, Genetic
Restriction Mapping

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