Article
De novo derivation of proteomes from transcriptomes for transcript and protein identification.
Nature methods - 1 Dec 2012
Evans Vanessa C, Barker Gary, Heesom Kate J, Fan Jun, Bessant Conrad, Matthews David A
Abstract excerpt
Identification of proteins by tandem mass spectrometry requires a reference protein database, but these are only available for model species. Here we demonstrate that, for a non-model species, the sequencing of expressed mRNA can generate a protein database for mass spectrometry-based identification. This combination of high-throughput sequencing and protein identification technologies allows detection of genes...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.