Article
In vitro transcription activities of Pol IV, Pol V, and RDR2 reveal coupling of Pol IV and RDR2 for dsRNA synthesis in plant RNA silencing.
Molecular cell - 14 Dec 2012
Haag Jeremy R, Ream Thomas S, Marasco Michelle, Nicora Carrie D, Norbeck Angela D, Pasa-Tolic Ljiljana, Pikaard Craig S
Abstract excerpt
In Arabidopsis, RNA-dependent DNA methylation and transcriptional silencing involves three nuclear RNA polymerases that are biochemically undefined: the presumptive DNA-dependent RNA polymerases Pol IV and Pol V and the putative RNA-dependent RNA polymerase RDR2. Here we demonstrate their RNA polymerase activities in vitro. Unlike Pol II, Pols IV and V require an RNA primer, are insensitive to α-amanitin, and...
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