Article
Building the repertoire of dispensable chromosome regions in Bacillus subtilis entails major refinement of cognate large-scale metabolic model.
Nucleic acids research - 7 Jan 2013
Tanaka Kosei, Henry Christopher S, Zinner Jenifer F, Jolivet Edmond, Cohoon Matthew P, Xia Fangfang, Bidnenko Vladimir, Ehrlich S Dusko, Stevens Rick L, Noirot Philippe
Abstract excerpt
The nonessential regions in bacterial chromosomes are ill-defined due to incomplete functional information. Here, we establish a comprehensive repertoire of the genome regions that are dispensable for growth of Bacillus subtilis in a variety of media conditions. In complex medium, we attempted deletion of 157 individual regions ranging in size from 2 to 159 kb. A total of 146 deletions were successful in complex...
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