Article
Modified method for combined DNA and RNA isolation from peanut and other oil seeds.
Molecular biology reports - 1 Feb 2013
Dang Phat M, Chen Charles Y
Abstract excerpt
Isolation of good quality RNA and DNA from seeds is difficult due to high levels of polysaccharides, polyphenols, and lipids that can degrade or co-precipitate with nucleic acids. Standard RNA extraction methods utilizing guanidinium-phenol-chloroform extraction has not shown to be successful. RNA isolation from plant seeds is a prerequisite for many seed specific gene expression studies and DNA is necessary in...
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