Article
Biolistics-based gene silencing in plants using a modified particle inflow gun.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Davies Kevin M, Deroles Simon C, Boase Murray R, Hunter Don A, Schwinn Kathy E
Abstract excerpt
RNA interference (RNAi) is one of the most commonly used techniques for examining the function of genes of interest. In this chapter we present two examples of RNAi that use the particle inflow gun for delivery of the DNA constructs. In one example transient RNAi is used to show the function of an anthocyanin regulatory gene in flower petals. In the second example stably transformed cell cultures are produced...
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