Article
Virus-Induced Gene Silencing for Functional Analysis of Flower Traits in Petunia.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020
Broderick Shaun R, Chapin Laura J, Jones Michelle L
Abstract excerpt
Virus-induced gene silencing (VIGS) uses recombinant viruses to knock down the expression of endogenous plant genes, allowing for rapid functional analysis without generating stable transgenic plants. The Tobacco rattle virus (TRV) is a popular vector for VIGS because it has a wide host range that includes Petunia × hybrida (petunia), and it induces minimal viral symptoms. Using reporter genes like chalcone...
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