Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Site-directed mutagenesis to study the role of specific amino acids in the ligand binding domain of PPARs.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

Mitro Nico, Gilardi Federica, Giudici Marco, Godio Cristina, Scotti Elena, Crestani Maurizio

Abstract excerpt

The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis, a technique broadly used...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acids
Share to Deoxyribonucleases, Type II Site-Specific
Share to HEK293 Cells

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Ligands

Share to Mutagenesis, Site-Directed

Share to Plasmids

Share to PPAR gamma

Share to Transformation, Genetic

Source

Identifiers

PMID: 23100229
DOI: 10.1007/978-1-62703-155-4_9

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-23100229-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Site-directed mutagenesis to study the role of specific amino acids in the ligand binding domain of PPARs.PMID 23100229Read ↗

Site-directed mutagenesis.Semantic proximity Read ↗

Site-directed mutagenesis in analysis of protein-protein interactions.Semantic proximity Read ↗

Single-Nucleotide Polymorphism of PPARγ, a Protein at the Crossroads of Physiological and Pathological Processes.Semantic proximity Read ↗

Substitution of a conserved amino acid residue alters the ligand binding properties of peroxisome proliferator activated receptors.Semantic proximity Read ↗

A database of mutants and effects of site-directed mutagenesis experiments on G protein-coupled receptors.Semantic proximity Read ↗

Alteration of a single amino acid in peroxisome proliferator-activated receptor-alpha (PPAR alpha) generates a PPAR delta phenotype.Semantic proximity Read ↗

Natural helix 9 mutants of PPARγ differently affect its transcriptional activitySemantic proximity Read ↗

Determination of the critical amino acids involved in the peroxisome proliferator-activated receptor (PPAR) delta selectivity of phenylpropanoic acid-derived agonists.Semantic proximity Read ↗

Alanine scanning mutational analysis of the ligand binding pocket of the human Vitamin D receptor.Semantic proximity Read ↗

[Mutagenesis study of pharmacological receptors: approach to their structure-function relationships].Semantic proximity Read ↗

Site-directed mutagenesis and its application to protein folding.Semantic proximity Read ↗

Mutation goals in the vitamin D receptor predicted by computational methods.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.