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Article

Human IgA-binding peptides selected from random peptide libraries: affinity maturation and application in IgA purification.

The Journal of biological chemistry - 14 Dec 2012

Hatanaka Takaaki, Ohzono Shinji, Park Mirae, Sakamoto Kotaro, Tsukamoto Shogo, Sugita Ryohei, Ishitobi Hiroyuki, Mori Toshiyuki, Ito Osamu, Sorajo Koichi, Sugimura Kazuhisa, Ham Sihyun, Ito Yuji

Abstract excerpt

Phage display system is a powerful tool to design specific ligands for target molecules. Here, we used disulfide-constrained random peptide libraries constructed with the T7 phage display system to isolate peptides specific to human IgA. The binding clones (A1-A4) isolated by biopanning exhibited clear specificity to human IgA, but the synthetic peptide derived from the A2 clone exhibited a low...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 23076147
DOI: 10.1074/jbc.M112.389742
PMCID: PMC3522307

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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