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18S rRNAis a reliable normalisation gene for real time PCR based on influenza virus infected cells

8 Oct 2012

Abstract excerpt

BACKGROUND: One requisite of quantitative reverse transcription PCR (qRT-PCR) is to normalise the data with an internal reference gene that is invariant regardless of treatment, such as virus infection. Several studies have found variability in the expression of commonly used housekeeping genes, such as beta-actin (ACTB) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), under different experimental settings....

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PMID: 23043930
DOI: 10.1186/1743-422x-9-230

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