Article
β-actin gene expression is variable among individuals and not suitable for normalizing mRNA levels in Portunus trituberculatus.
Fish & shellfish immunology - 1 Oct 2018
Zhou Su-Ming, Tao Zhen, Shen Chen, Qian Dong, Wang Chun-Lin, Zhou Qi-Cun, Jin Shan
Abstract excerpt
The housekeeping gene encoding β-actin appears to be the most widely-used internal reference for gene expression studies in experimental animals or their cell lines. However, the effectiveness of β-actin to normalize mRNA levels expression in many crustacean species is still object of debate. To date, it is still unclear if β-actin is suitable to be utilized as the internal reference in qualitative real-time gene...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
