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Article

Influence of heat stress on reference genes stability in heart and liver of two chickens genotypes.

PloS one - 1 Jan 2020

Gromboni Juliana Gracielle Gonzaga, Cedraz de Oliveira Haniel, Botelho Diniz Marques Daniele, Amândio Pinto Garcia Junior Antônio, Vasconcelos Farias Filho Ronaldo, Fernando Gromboni Caio, Machado Souza Teillor, Arias Wenceslau Amauri

Abstract excerpt

INTRODUCTION: Real-time polymerase chain reaction (RT-qPCR) is an important tool for analyzing gene expression. However, before analyzing the expression of target genes, it is crucial to normalize the reference genes, in order to find the most stable gene to be used as an endogenous control. A gene that remains stable in all samples under different treatments is considered a suitable normalizer. In this sense, we...

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PMID: 32027666
DOI: 10.1371/journal.pone.0228314
PMCID: PMC7004300

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