A Universal Avian Endogenous Real-Time Reverse Transcriptase–Polymerase Chain Reaction Control and Its Application to Avian Influenza Diagnosis and Quantification

Abstract excerpt

Real-time reverse transcriptase-polymerase chain reaction (RRT-PCR) is becoming an established first-line diagnostic assay as well as a precise quantification tool for avian influenza virus detection. However, there remain some limitations. First, we show that the sensitivity of RRT-PCR influenza detection can be 10- to 100-fold inhibited in oropharyngeal and cloacal swabs. Adding 0.5 U of heat-activated Taq DNA...

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