Article
Quality requirements for genomic DNA preparations and storage conditions for a high-density oligonucleotide microarray.
Biological & pharmaceutical bulletin - 1 Jan 2012
Kurose Kouichi, Koizumi Tomoko, Nishikawa Jun, Maekawa Keiko, Saito Yoshiro
Abstract excerpt
High-density oligonucleotide microarrays are widely used in genome-wide association studies. The purpose of this study was to assess the influence of various factors during the preparation of DNA on genotype calling for the Affymetrix high-density oligonucleotide microarray 250K GeneChip. DNA was extracted from peripheral whole blood by solution-based and silica-membrane-based methods. Blood was stored at 4°C or...
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