Article
Role of protons in sugar binding to LacY.
Proceedings of the National Academy of Sciences of the United States of America - 16 Oct 2012
Smirnova Irina, Kasho Vladimir, Sugihara Junichi, Vázquez-Ibar José Luis, Kaback H Ronald
Abstract excerpt
WT lactose permease of Escherichia coli (LacY) reconstituted into proteoliposomes loaded with a pH-sensitive fluorophore exhibits robust uphill H(+) translocation coupled with downhill lactose transport. However, galactoside binding by mutants defective in lactose-induced H(+) translocation is not accompanied by release of an H(+) on the interior of the proteoliposomes. Because the pK(a) value for galactoside...
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