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Article

Uncoupling in secondary transport proteins. A mechanistic explanation for mutants of lac permease with an uncoupled phenotype.

The Journal of biological chemistry - 26 May 1995

Lolkema J S, Poolman B

Abstract excerpt

The kinetic behavior of a H(+)-substrate symporter has been studied in which in addition to the unloaded (E) and fully loaded states (E.S.H) of the carrier also one of the binary complexes (E.S or E.H) may reorient its binding sites. This results in two types of uncoupled mutants, the ES leak and the EH leak type. The effects of pH and substrate concentration (pS) on the coupling of transport have been analyzed....

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Source

Identifiers

PMID: 7759518
DOI: 10.1074/jbc.270.21.12670

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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