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Article

Probing of the substrate binding domain of lactose permease by a proton pulse.

Biochimica et biophysica acta - 3 Sept 2001

Nachliel E, Gutman M

Abstract excerpt

The lactose permease of Escherichia coli coupled proton transfer across the bacterial inner membrane with the uptake of beta-galactosides. In the present study we have used the cysteine-less C148 mutant that was selectively labeled by fluorescein maleimide on the C148 residue, which is an active component of the substrate transporting cavity. Measurements of the protonation dynamics of the bound pH indicator in...

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PMID: 11513803
DOI: 10.1016/s0005-2736(01)00361-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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