Article
In vitro translation and processing of cathepsin B of Schistosoma mansoni.
The EMBO journal - 1 Feb 1990
Felleisen R, Klinkert M Q
Abstract excerpt
Cathepsin B has been implicated with a key role in schistosome metabolism. Here, we investigate the processing of preprocathepsin B to the mature enzyme in in vitro transcription and translation experiments. The cDNA sequence encoding the precursor of cathepsin B of Schistosoma mansoni was cloned in a modified RNA expression vector pSP65 and the derived RNA expressed in rabbit reticulocyte lysates. Transcripts...
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