Article
Redesigning and characterizing the substrate specificity and activity of Vibrio fluvialis aminotransferase for the synthesis of imagabalin.
Protein engineering, design & selection : PEDS - 1 Jan 2013
Midelfort Katarina S, Kumar Rajesh, Han Seungil, Karmilowicz Michael J, McConnell Kevin, Gehlhaar Daniel K, Mistry Anil, Chang Jeanne S, Anderson Marie, Villalobos Alan, Minshull Jeremy, Govindarajan Sridhar, Wong John W
Abstract excerpt
Several protein engineering approaches were combined to optimize the selectivity and activity of Vibrio fluvialis aminotransferase (Vfat) for the synthesis of (3S,5R)-ethyl 3-amino-5-methyloctanoate; a key intermediate in the synthesis of imagabalin, an advanced candidate for the treatment of generalized anxiety disorder. Starting from wild-type Vfat, which had extremely low activity catalyzing the desired...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.