Article
Role of the ribosomal protein L27 revealed by single-molecule FRET study.
Protein science : a publication of the Protein Society - 1 Nov 2012
Wang Yuhong, Xiao Ming
Abstract excerpt
The ribosome is a ribozyme. However, in bacterial ribosomes, the N-terminus of L27 is located within the peptidyl transfer center. The roles of this protein in real time remain unclear. We present single-molecule fluorescence resonance energy transfer (FRET) studies of tRNA dynamics at the peptidyl transfer center in ribosomes containing either wild type (WT) L27, or L27 mutants with A2H3, A2H3K4 or nine...
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