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[Construction of ectoine absorption defective mutant for efficient ectoine production].

Wei sheng wu xue bao = Acta microbiologica Sinica - 4 May 2012

Xu Rui, Zhang Linghua

Abstract excerpt

OBJECTIVE: To further enhance ectoine (1, 4, 5, 6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) synthesis efficiency. METHODS: We cloned Halomonas salina DSM 5928(T) teaABC gene of ectoine-specific transporter TeaABC by walking PCR and constructed ectoine absorption defective mutant H. salina DSM 5928(T) (teaABC-) by Red recombination technology. RESULTS: The total concentration of ectoine and productivity in...

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PMID: 22803353

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