Article
Isolation and characterization of salt-sensitive mutants of the moderate halophile Halomonas elongata and cloning of the ectoine synthesis genes.
The Journal of biological chemistry - 10 Oct 1997
Cánovas D, Vargas C, Iglesias-Guerra F, Csonka L N, Rhodes D, Ventosa A, Nieto J J
Abstract excerpt
The moderate halophile Halomonas elongata Deustche Sammlung für Mikroorganismen 3043 accumulated ectoine, hydroxyectoine, glutamate, and glutamine in response to osmotic stress (3 M NaCl). Two Tn1732-induced mutants, CHR62 and CHR63, that were severely affected in their salt tolerance were isolat...
Topics
- Amino Acids, Diamino
- Cloning, Molecular
- Halobacteriaceae
- Hydro-Lyases
- Magnetic Resonance Spectroscopy
- Models, Chemical
- Mutagenesis, Site-Directed
- Phenotype
- Transaminases