Article
Reconstituted basement membrane enhances neurite outgrowth in PC12 cells induced by nerve growth factor.
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research - 1 Jul 1990
Schwarz M A, Mitchell M, Emerson D L
Abstract excerpt
Rat pheochromocytoma cells (PC12) respond to nerve growth factor (NGF) by elaborating neurites. We have studied the effect of extracellular matrix proteins on responsiveness of PC12 cells to NGF. NGF elicited neurite outgrowth in cells plated on collagen, fibronectin, or laminin within 48-72 h. In contrast, cells entrapped in reconstituted basement membrane prepared from the Engelbreth-Holm-Swarm tumor (EHS-BM)...
Topics
- Adrenal Gland Neoplasms
- Animals
- Basement Membrane
- Cell Differentiation
- Collagen
- Culture Techniques
- Fibronectins
- Laminin
- Nerve Growth Factors
- Phenotype
- Pheochromocytoma