Article
I-SceI-mediated double-strand break does not increase the frequency of homologous recombination at the Dct locus in mouse embryonic stem cells.
PloS one - 1 Jan 2012
Fenina Myriam, Simon-Chazottes Dominique, Vandormael-Pournin Sandrine, Soueid Jihane, Langa Francina, Cohen-Tannoudji Michel, Bernard Bruno A, Panthier Jean-Jacques
Abstract excerpt
Targeted induction of double-strand breaks (DSBs) at natural endogenous loci was shown to increase the rate of gene replacement by homologous recombination in mouse embryonic stem cells. The gene encoding dopachrome tautomerase (Dct) is specifically expressed in melanocytes and their precursors....
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