Article
Large-scale development of cost-effective SNP marker assays for diversity assessment and genetic mapping in chickpea and comparative mapping in legumes.
Plant biotechnology journal - 1 Aug 2012
Hiremath Pavana J, Kumar Ashish, Penmetsa Ramachandra Varma, Farmer Andrew, Schlueter Jessica A, Chamarthi Siva K, Whaley Adam M, Carrasquilla-Garcia Noelia, Gaur Pooran M, Upadhyaya Hari D, Kavi Kishor Polavarapu B, Shah Trushar M, Cook Douglas R, Varshney Rajeev K
Abstract excerpt
A set of 2486 single nucleotide polymorphisms (SNPs) were compiled in chickpea using four approaches, namely (i) Solexa/Illumina sequencing (1409), (ii) amplicon sequencing of tentative orthologous genes (TOGs) (604), (iii) mining of expressed sequence tags (ESTs) (286) and (iv) sequencing of candidate genes (187). Conversion of these SNPs to the cost-effective and flexible throughput Competitive Allele Specific...
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