Article
Large-scale development of cost-effective single-nucleotide polymorphism marker assays for genetic mapping in pigeonpea and comparative mapping in legumes.
DNA research : an international journal for rapid publication of reports on genes and genomes - 1 Dec 2012
Saxena Rachit K, Penmetsa R Varma, Upadhyaya Hari D, Kumar Ashish, Carrasquilla-Garcia Noelia, Schlueter Jessica A, Farmer Andrew, Whaley Adam M, Sarma Birinchi K, May Gregory D, Cook Douglas R, Varshney Rajeev K
Abstract excerpt
Single-nucleotide polymorphisms (SNPs, >2000) were discovered by using RNA-seq and allele-specific sequencing approaches in pigeonpea (Cajanus cajan). For making the SNP genotyping cost-effective, successful competitive allele-specific polymerase chain reaction (KASPar) assays were developed for 1616 SNPs and referred to as PKAMs (pigeonpea KASPar assay markers). Screening of PKAMs on 24 genotypes [23 from...
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