Article
Elimination of a reactive thiol group from the active site of chloramphenicol acetyltransferase.
The Biochemical journal - 1 Dec 1990
Lewendon A, Shaw W V
Abstract excerpt
1. The type III variant of chloramphenicol acetyltransferase (CATIII) is resistant to inactivation by ionizable modifying reagents such as 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) and iodoacetate, whereas it is sensitive to inhibition by similar but uncharged reagents, including 4,4'-dithiodipyridine, methyl methanethiolsulphonate (MMTS) and iodoacetamide. The target for these thiol-modifying reagents has been...
Topics
- Base Sequence
- Binding Sites
- Chloramphenicol O-Acetyltransferase
- Dithionitrobenzoic Acid
- Escherichia coli
- Genetic Variation
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligonucleotide Probes