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Article

Fluorinated chloramphenicol acetyltransferase thermostability and activity profile: improved thermostability by a single-isoleucine mutant.

Bioorganic & medicinal chemistry letters - 1 Nov 2007

Voloshchuk Natalya, Lee Man Xia, Zhu Wan Wen, Tanrikulu Ismet Caglar, Montclare Jin Kim

Abstract excerpt

A lysate-based thermostability and activity profile is described for chloramphenicol acetyltransferase (CAT) expressed in trifluoroleucine, T (CAT T). CAT and 13 single-isoleucine CAT mutants were expressed in medium supplemented with T and assayed for thermostability on cell lysates. Although fluorinated mutants, L82I T and L208I T, showed losses in thermostability, the L158I T fluorinated mutant demonstrated an...

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Identifiers

PMID: 17845847
DOI: 10.1016/j.bmcl.2007.07.107

Genes

CAT

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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