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Article

High resolution MICA genotyping by sequence-based typing (SBT).

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012

Zou Yizhou, Stastny Peter

Abstract excerpt

We have developed a MICA typing method based on polymerase chain reaction (PCR) sequence-based typing and a computer program that determines the polymorphisms and distinguishes the GCT repeats in exon 5. One PCR amplification was performed to obtain templates of 2.2 kb, including exons 2, 3, 4, and 5 of MICA to be sequenced with two forward and two reverse primers. Overlay of nucleotide sequencing signals...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 22665235
DOI: 10.1007/978-1-61779-842-9_11

Genes

MICA

Provenance

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