Article
A measurable activation of the bZIP transcription factor Atf1 in a fission yeast strain devoid of stress-activated and cell integrity mitogen-activated protein kinase (MAPK) activities.
The Journal of biological chemistry - 6 Jul 2012
Zhou Xin, Ma Yan, Kato Toshiaki, Kuno Takayoshi
Abstract excerpt
In Schizosaccharomyces pombe, the stress-activated Sty1 MAPK pathway is essential for cell survival under stress conditions. The Sty1 MAPK regulates Atf1 transcription factor to elicit stress responses in extreme conditions of osmolarity and reactive oxygen species-generating agents such as hydrogen peroxide, heat, low glucose, and heavy metal. Herein, using a newly developed Renilla luciferase reporter assay...
Topics
- Activating Transcription Factor 1
- Cadmium Chloride
- Hydrogen Peroxide
- Luciferases
- Mitogen-Activated Protein Kinases
- Mutation
- Oxidants
- Phosphoproteins
- Potassium Chloride
- Reverse Transcriptase Polymerase Chain Reaction
- Schizosaccharomyces
- Schizosaccharomyces pombe Proteins