Article
Low nanomolar GABA effects at extrasynaptic α4β1/β3δ GABA(A) receptor subtypes indicate a different binding mode for GABA at these receptors.
Biochemical pharmacology - 15 Aug 2012
Karim Nasiara, Wellendorph Petrine, Absalom Nathan, Bang Line Haunstrup, Jensen Marianne Lerbech, Hansen Maja Michelle, Lee Ho Joon, Johnston Graham A R, Hanrahan Jane R, Chebib Mary
Abstract excerpt
Ionotropic GABA(A) receptors are a highly heterogenous population of receptors assembled from a combination of multiple subunits. The aims of this study were to characterize the potency of GABA at human recombinant δ-containing extrasynaptic GABA(A) receptors expressed in Xenopus oocytes using the two-electrode voltage clamp technique, and to investigate, using site-directed mutagenesis, the molecular...
Topics
- Amino Acid Sequence
- Animals
- Female
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Oocytes
- Patch-Clamp Techniques
- Protein Subunits