Article
An Expanded Transposon Mutant Library Reveals that Vibrio fischeri δ-Aminolevulinate Auxotrophs Can Colonize Euprymna scolopes.
Applied and environmental microbiology - 1 Mar 2017
Lyell Noreen L, Septer Alecia N, Dunn Anne K, Duckett Drew, Stoudenmire Julie L, Stabb Eric V
Abstract excerpt
Libraries of defined mutants are valuable research tools but necessarily lack gene knockouts that are lethal under the conditions used in library construction. In this study, we augmented a Vibrio fischeri mutant library generated on a rich medium (LBS, which contains [per liter] 10 g of tryptone, 5 g of yeast extract, 20 g of NaCl, and 50 mM Tris [pH 7.5]) by selecting transposon insertion mutants on...
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