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Article

Measurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactions.

Methods in enzymology - 1 Jan 2012

Slutzki Michal, Barak Yoav, Reshef Dan, Schueler-Furman Ora, Lamed Raphael, Bayer Edward A

Abstract excerpt

The cellulosome is a large bacterial extracellular multienzyme complex able to degrade crystalline cellulosic substrates. The complex contains catalytic and noncatalytic subunits, interconnected by high-affinity cohesin-dockerin interactions. In this chapter, we introduce an optimized method for comparative binding among different cohesins or cohesin mutants to the dockerin partner. This assay offers advantages...

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PMID: 22608739
DOI: 10.1016/B978-0-12-415931-0.00022-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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Measurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactions.PMID 22608739Read ↗

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