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Article

Simultaneous evaluation of six human glucuronidation activities in liver microsomes using liquid chromatography-tandem mass spectrometry.

Analytical biochemistry - 1 Aug 2012

Gagez Anne-Laure, Rouguieg-Malki Koukeb, Sauvage François-Ludovic, Marquet Pierre, Picard Nicolas

Abstract excerpt

This article describes the development of a procedure for the simultaneous evaluation of the activity of six different uridine diphosphate (UDP)-glucuronyltransferases (UGTs) in human liver microsomes (HLMs). The method consists of incubations of probe substrates for UGT1A1 (etoposide), UGT1A3 (chenodeoxycholic acid), UGT1A4 (trifluoperazine), UGT1A6 (serotonin), UGT1A9 (mefenamic acid), and UGT2B7...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 22579593
DOI: 10.1016/j.ab.2012.04.031

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