Article
Simultaneous amplification and identification of 25 human papillomavirus types with Templex technology.
Journal of clinical microbiology - 1 Nov 2006
Han Jian, Swan David C, Smith Sharon J, Lum Shanjuan H, Sefers Susan E, Unger Elizabeth R, Tang Yi-Wei
Abstract excerpt
The majority of existing human papillomavirus (HPV) genotyping assays are based on multiplex PCR using consensus or degenerate primers. We developed a Templex HPV assay that simultaneously detects and identifies 25 common HPV genotypes in a single-tube reaction using type-specific primers for the HPV-specific E6 and E7 genes. The analytical sensitivities of the Templex assay for HPV type 16 (HPV-16), -18, and -56...
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