Nucleotide sequence and expression of the isoamylase gene from an isoamylase-hyperproducing mutant, Pseudomonas amyloderamosa JD210.

doi:10.1016/0167-4781(90)90004-l

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Article

Nucleotide sequence and expression of the isoamylase gene from an isoamylase-hyperproducing mutant, Pseudomonas amyloderamosa JD210.

Biochimica et biophysica acta - 30 Nov 1990

Chen J H, Chen Z Y, Chow T Y, Chen J C, Tan S T, Hsu W H

Abstract excerpt

The isoamylase gene (ISO) of Pseudomonas amyloderamosa JD210, an isoamylase-hyperproducing mutant, was cloned in an isoamylase-deficient and transformable mutant strain K31. By deletion analysis, the ISO gene was found to be located within a 3.3 kilobases BamHI fragment. Its nucleotide sequence c...

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Bacterial
Escherichia coli
Genes, Bacterial
Isoamylase
Molecular Sequence Data
Mutation
Plasmids
Pseudomonas
Restriction Mapping

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