Transcriptional analysis of the promoter region of the Pseudomonas putida branched-chain keto acid dehydrogenase operon.

doi:10.1128/jb.172.10.5655-5663.1990

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Article

Transcriptional analysis of the promoter region of the Pseudomonas putida branched-chain keto acid dehydrogenase operon.

Journal of bacteriology - 1 Oct 1990

Madhusudhan K T, Huang G, Burns G, Sokatch J R

Abstract excerpt

Branched-chain keto acid dehydrogenase is a multienzyme complex produced by Pseudomonas putida when it is grown in a minimal medium containing branched-chain amino acids. A 1.87-kilobase (kb) DNA fragment was cloned and sequenced which contained 0.24 kb of the E1 alpha structural gene and 1.6 kb of upstream DNA. There were 854 base pairs (bp) of noncoding DNA upstream of bkdA1, the first gene of the bkd operon,...

Topics

3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Base Sequence
Chromosome Deletion
Cloning, Molecular
Genotype
Ketone Oxidoreductases
Molecular Sequence Data
Multienzyme Complexes
Operon
Phenotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasmids

Promoter Regions, Genetic

Pseudomonas

Restriction Mapping

Sequence Homology, Nucleic Acid

Transcription, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 2211503
DOI: 10.1128/jb.172.10.5655-5663.1990
PMCID: PMC526879

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2211503-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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